ip3r inhibitor 2-apb  (ApexBio)

Journal: Experimental eye research

Article Title: Differential mitochondrial and cellular responses between H vs. J mtDNA haplogroup-containing human RPE transmitochondrial cybrid cells

doi: 10.1016/j.exer.2022.109013

Figure Lengend Snippet: A. J and H cybrids pre-treated with an IP3R inhibitor, images show improved mitochondrial network and cellular distribution in J cybrids after 30 min of oxidative stress (20x). Scale bar = 10 μM. B. Higher magnification images (100x) show the structural changes before and after IP3Ri in stressed condition. (green: TOMM20; red: actin; blue: dapi; n = 6). Scale bar = 20 μM.

Article Snippet: IP3R inhibition/Flow cytometry 2-Aminoethoxydiphenyl borate (2-APB) (Santa Cruz Biotechnology, sc-201487) was used to inhibit the inositol 1,4,5-triphosphate receptor (IP3R).

Techniques:

Journal: Experimental eye research

Article Title: Differential mitochondrial and cellular responses between H vs. J mtDNA haplogroup-containing human RPE transmitochondrial cybrid cells

doi: 10.1016/j.exer.2022.109013

Figure Lengend Snippet: A. The flow cytometry data showed that treatment with the IP3R inhibitor, 2-Aminoethoxydiphenyl borate (2APB) decreased [Ca2+]m under stressed conditions by 3.74-fold (n = 6; p < 0.001) in J cybrids as well as in H cybrids with 1.57-fold changes (n = 6; p < 0.001). B. Both RT and q-PCR showed that levels of expression of IP3R and MCU, which play a role in [Ca2+]m, are decreased by 1.60 and 1.51-fold (n = 3; p value; H is set at 1; ***p < 0.001). The brown bars show negative regulation, and the white bars show positive regulation.

Article Snippet: IP3R inhibition/Flow cytometry 2-Aminoethoxydiphenyl borate (2-APB) (Santa Cruz Biotechnology, sc-201487) was used to inhibit the inositol 1,4,5-triphosphate receptor (IP3R).

Techniques: Flow Cytometry, Expressing

Journal: Frontiers in immunology

Article Title: Inhibition of IP3R/Ca2+ Dysregulation Protects Mice From Ventilator-Induced Lung Injury via Endoplasmic Reticulum and Mitochondrial Pathways.

doi: 10.3389/fimmu.2021.729094

Figure Lengend Snippet: FIGURE 3 | Effects of carbachol and 2-APB on HTV-induced Ca2+ homeostasis in lungs. Single-cell suspensions from lung tissues were preloaded with Fluo-4 AM, Rhod-2 and Mag-Fluo-AM, respectively. (A) Fluo-4 AM labeling the cytoplasmic Ca2+ in lung tissues from CON group, HTV group, IP3R agonist carbachol pretreatment upon HTV stimulation group (HTV+Carbachol group) and IP3R inhibitor 2-APB pretreatment upon HTV stimulation group (HTV+2-APB group). An inset picture was employed to show the indicated area at 4X magnification. (B) Fluorescence quantification analysis of Fluo-4 AM. (C) Rhod-2 and Mag-Fluo-AM co- dyeing to label Ca2+ in the mitochondria and ER in different groups. An inset picture was employed to show the indicated area at 4X magnification. (D) Fluorescence quantification analysis of Rhod-2. (E) Fluorescence quantification analysis of Mag-Fluo-AM. Data are expressed as means ± SD (n = 6 per group). *P < 0.05 vs. CON group; #P < 0.05 vs. HTV group.

Article Snippet: Reagents used in this study included IP3R agonist carbachol (Abmole Bioscience,USA), IP3R inhibitor 2-aminoethoxydiphenyl borate (2-APB, Tocris Biosciences, UK), and Ca2+ chelator September 2021 | Volume 12 | Article 729094 BAPTA-AM (MedChemExpress, USA).

Techniques: Labeling, Fluorescence

Journal: Frontiers in immunology

Article Title: Inhibition of IP3R/Ca2+ Dysregulation Protects Mice From Ventilator-Induced Lung Injury via Endoplasmic Reticulum and Mitochondrial Pathways.

doi: 10.3389/fimmu.2021.729094

Figure Lengend Snippet: FIGURE 12 | Working model detailing the role of IP3R/Ca2+ in VILI. Mechanical overstretch induces cell inflammation through an accelerated ER-to-cytosolic and ER-to-mitochondria efflux of Ca2+ through IP3R, followed by ER stress and mitochondrial dysfunction.

Article Snippet: Reagents used in this study included IP3R agonist carbachol (Abmole Bioscience,USA), IP3R inhibitor 2-aminoethoxydiphenyl borate (2-APB, Tocris Biosciences, UK), and Ca2+ chelator September 2021 | Volume 12 | Article 729094 BAPTA-AM (MedChemExpress, USA).

Techniques: